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Production of plasma membrane vesicles with chloride salts and their utility as a cell membrane mimetic for biophysical characterization of membrane protein interactions. Anal Chem · Oct 16, 2012

  • Foto del escritor: Carolina Agudelo Arbeláez
    Carolina Agudelo Arbeláez
  • 16 oct 2012
  • 1 Min. de lectura
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This article introduces a novel, artifact-free method to generate plasma membrane vesicles from CHO cells using a hypertonic buffer without formaldehyde or DTT. The approach is validated through size distribution analysis and its effectiveness in studying membrane protein interactions is demonstrated.



Continue reading on ACS Publications: https://pubs.acs.org/doi/abs/10.1021/ac301776j


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